100 110 120 M-F RTFPGIPKWR KTHLTYRIVN 130 140 150 160 170 180 YTPDLPKDAV DSAVEKALKV WEEVTPLTFS RLYEGEADIM ISFAVREHGD DYPFDGPGNV 190 200 210 220 230 240 LAHAYAPGPG INGDAHFDDD EQWTKDTTGT NLFLVAAHEI GHSLGLFHSA NTEALMYPLY 250 260 265 HSLTDLTRFR LSQDDINGIQ SLYGP
Purity > 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as a single band migrating at a molecular weight between 18.4 and 25.0 kDa.
Suppied As 0.2 mg/mL solution in Tris 20 mM, pH 7.2, CaCl2 10 mM, ZnCl2 0.1 mM, NaCl 0.3 M, acetohydroxamic acid (AHA) 0.2 M. The concentration is calculated by the analysis of the absorbance at 280 nm (ε280 = 28420 M-1cm-1 calculated).
Specific activity > 30 U/μg. Activity described as U=100 pmol/min at 37°C using a colorimetric ***** with thiopeptolide Ac-Pro-Leu-Gly-[2- mercapto-4-methyl-pentanoyl]-Leu-Gly-OC2H5 (Biomol) as substrate.
Storage -80°C. After initial defrost, aliquot the product into individual tubes and refreeze at -80°C. Avoid repeated freeze/thaw cycles.